Nucleic acids
Carriers of genetic information in living organisms
Prokaryotes
Organisms that package DNA into circular chromosomes in the nucleoid
Eukaryotes
Organisms that package DNA into linear chromosomes in the nucleus
Plasmids
Small
Extranuclear DNA
Genetic material found in plasmids outside the nucleus
Nucleotides
Consist of a nitrogenous base
Purines
Nitrogenous bases with a two-ringed structure (adenine and guanine)
Pyrimidines
Nitrogenous bases with a one-ringed structure (cytosine
Base-pairing rules
In DNA: A-T
Deoxyribose
Five-carbon sugar in DNA with a hydrogen at the 2' carbon
Ribose
Five-carbon sugar in RNA with a hydroxyl group at the 2' carbon
Semiconservative DNA replication
Each new double helix contains one original and one newly synthesized strand
Helicase
Enzyme that unwinds DNA strands at the origin of replication
Topoisomerase
Enzyme that relieves supercoiling by making nicks in DNA backbone
DNA polymerase
Enzyme adding nucleotides in 5' to 3' direction
Antiparallel
DNA strands oriented in opposite directions
Leading strand
Continuous DNA synthesis in 5' to 3' direction
Lagging strand
Discontinuous DNA synthesis in short fragments
Ligase
Enzyme connecting DNA fragments via phosphodiester bond
Semiconservative
DNA replication conserves one original and one new strand
RNA polymerase
Enzyme transcribing DNA into RNA molecules
Transcription
Process copying DNA into RNA by RNA polymerase
Translation
Process converting mRNA into proteins by ribosomes
Promoter
Noncoding DNA sequence binding RNA polymerase to start transcription
Codons
Three-base pair sequences on mRNA specifying amino acids
Template Strand
Also known as the 'minus strand' or antisense strand in DNA.
Pre-mRNA
Initial mRNA transcript in eukaryotic cells before modifications.
Introns
Noncoding RNA sequences in eukaryotic pre-mRNAs.
Exons
Coding sequences of eukaryotic RNAs.
Spliceosomes
Structures removing introns and joining exons in pre-mRNA.
5' Cap
Added to mRNA to protect ends and aid in translation initiation.
Poly-A Tail
String of adenine nucleotides added to the 3' end of pre-mRNA.
Alternative Splicing
Process generating multiple RNA transcripts from one gene.
Mature mRNA
Pre-mRNA after modifications
Ribosomes
Cellular structures where translation of mRNA occurs.
Initiation of Translation
Start of translation process with rRNA pairing with start codon.
Elongation of Polypeptide Chain
Continuation of polypeptide formation during translation.
Termination of Translation
End of translation process releasing the polypeptide chain.
Redundancy in the genetic code
Multiple codons can code for the same amino acid
Silent mutations
Mutations that do not change the amino acid sequence
Common ancestry
Shared genetic code among organisms as evidence
Codon chart
Provides correspondence between codons and amino acids
mRNA
Messenger RNA carries genetic information for translation
Ribosome small subunit
Part of ribosome where translation initiation occurs
Amino acid codons
Sequences that code for specific amino acids
Translation initiation
Start of protein synthesis on ribosome
Peptide bond
Chemical bond between amino acids in a protein
Elongation
Process of adding amino acids to a growing polypeptide chain
Termination
End of translation when a stop codon is reached
Release factors
Proteins that trigger ribosome disassembly at stop codons
Reverse transcriptase
Enzyme in retroviruses that makes DNA from RNA
Regulatory proteins
Proteins that control gene expression by binding to specific nucleotide sequences
Regulatory sequences
Nucleotide sequences where regulatory proteins bind to control gene expression
Mutations
Changes in the genome that can impact gene expression
Operon
Cluster of genes with a common function controlled by a common promoter
Operator
Noncoding sequence binding repressor proteins to regulate transcription
Structural genes
Coding sequences containing genetic information for operon function
Inducible operons
Operons activated by inducer molecules to perform specific functions
Repressible operons
Operons that are normally active but can be inhibited by excess product
Catabolic function
Operons involved in breaking down molecules for energy
Corepressor
Molecule that binds to a repressor to inhibit transcription
Cyclic AMP (cAMP)
Molecule whose interaction with CAP can upregulate gene expression
Catabolite activator protein (CAP)
Protein that binds cAMP to stimulate transcription near the promoter
trp repressor protein
Protein that binds to tryptophan before binding to the operator
tryptophan
Amino acid that binds to trp repressor
tor
RNA polymerase that transcribes the operon
corepressor
Molecule that binds to repressor
Promoters (Eukaryotes)
Binding sites for RNA polymerase in eukaryotic gene regulation
Enhancers
Regulatory switches where activator proteins bind in eukaryotes
Silencers
Regulatory switches where repressor proteins bind in eukaryotes
Epigenetic changes
Reversible modifications to DNA affecting gene expression in eukaryotes
Hox genes
Family of genes that code for transcription factors
Antennapedia
Hox gene controlling leg formation in Drosophila
Phenotype
Observable traits resulting from gene expression
Natural selection
Process where beneficial traits are favored for survival
Aneuploidy
Condition with an incorrect number of chromosomes
Klinefelter syndrome
Genetic disorder with XXY sex chromosomes
Down syndrome
Genetic disorder with an extra copy of chromosome 21
Polyploids
Organisms with an entire extra set of chromosomes
Horizontal transmission
Transfer of genetic information between same-generation members
Transformation
Uptake of foreign DNA by a cell
Transduction
DNA transmission between organisms by viruses
Conjugation
DNA transmission through cell-to-cell contact
Transposition
Movement of DNA between or within chromosomes
Bacterial Transformation
Introduction of foreign DNA into bacterial cells
Recombinant DNA
DNA recombined from different source organisms
Restriction Endonucleases
Enzymes cutting DNA at specific sequences
DNA Ligases
Enzymes connecting DNA fragments
Recombinant Plasmids
Plasmids with selectable markers and desired gene
Gel Electrophoresis
Separates DNA fragments by size and charge
Agarose Gel
Matrix for DNA migration in gel electrophoresis
Polymerase Chain Reaction (PCR)
Amplifies specific DNA fragments
Denaturing
Separates DNA strands in PCR
Annealing
Hydrogen bonding of primers in PCR
Extension
DNA polymerase adds nucleotides in PCR
CRISPR-Cas9
System editing DNA sequences using guide RNA
Knockout
Disabling a gene's function using CRISPR
DNA Sequencing
Determining the order of nucleotides in DNA
Bacteriophages
Viruses infecting bacteria
Guide RNA
Directs Cas9 to cut at specific DNA sequences