AP Bio: Unit 6

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Nucleic acids

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100 Terms

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Nucleic acids

Carriers of genetic information in living organisms

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Prokaryotes

Organisms that package DNA into circular chromosomes in the nucleoid

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Eukaryotes

Organisms that package DNA into linear chromosomes in the nucleus

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Plasmids

Small

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Extranuclear DNA

Genetic material found in plasmids outside the nucleus

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Nucleotides

Consist of a nitrogenous base

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Purines

Nitrogenous bases with a two-ringed structure (adenine and guanine)

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Pyrimidines

Nitrogenous bases with a one-ringed structure (cytosine

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Base-pairing rules

In DNA: A-T

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Deoxyribose

Five-carbon sugar in DNA with a hydrogen at the 2' carbon

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Ribose

Five-carbon sugar in RNA with a hydroxyl group at the 2' carbon

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Semiconservative DNA replication

Each new double helix contains one original and one newly synthesized strand

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Helicase

Enzyme that unwinds DNA strands at the origin of replication

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Topoisomerase

Enzyme that relieves supercoiling by making nicks in DNA backbone

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DNA polymerase

Enzyme adding nucleotides in 5' to 3' direction

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Antiparallel

DNA strands oriented in opposite directions

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Leading strand

Continuous DNA synthesis in 5' to 3' direction

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Lagging strand

Discontinuous DNA synthesis in short fragments

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Ligase

Enzyme connecting DNA fragments via phosphodiester bond

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Semiconservative

DNA replication conserves one original and one new strand

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RNA polymerase

Enzyme transcribing DNA into RNA molecules

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Transcription

Process copying DNA into RNA by RNA polymerase

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Translation

Process converting mRNA into proteins by ribosomes

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Promoter

Noncoding DNA sequence binding RNA polymerase to start transcription

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Codons

Three-base pair sequences on mRNA specifying amino acids

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Template Strand

Also known as the 'minus strand' or antisense strand in DNA.

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Pre-mRNA

Initial mRNA transcript in eukaryotic cells before modifications.

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Introns

Noncoding RNA sequences in eukaryotic pre-mRNAs.

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Exons

Coding sequences of eukaryotic RNAs.

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Spliceosomes

Structures removing introns and joining exons in pre-mRNA.

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5' Cap

Added to mRNA to protect ends and aid in translation initiation.

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Poly-A Tail

String of adenine nucleotides added to the 3' end of pre-mRNA.

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Alternative Splicing

Process generating multiple RNA transcripts from one gene.

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Mature mRNA

Pre-mRNA after modifications

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Ribosomes

Cellular structures where translation of mRNA occurs.

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Initiation of Translation

Start of translation process with rRNA pairing with start codon.

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Elongation of Polypeptide Chain

Continuation of polypeptide formation during translation.

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Termination of Translation

End of translation process releasing the polypeptide chain.

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Redundancy in the genetic code

Multiple codons can code for the same amino acid

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Silent mutations

Mutations that do not change the amino acid sequence

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Common ancestry

Shared genetic code among organisms as evidence

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Codon chart

Provides correspondence between codons and amino acids

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mRNA

Messenger RNA carries genetic information for translation

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Ribosome small subunit

Part of ribosome where translation initiation occurs

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Amino acid codons

Sequences that code for specific amino acids

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Translation initiation

Start of protein synthesis on ribosome

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Peptide bond

Chemical bond between amino acids in a protein

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Elongation

Process of adding amino acids to a growing polypeptide chain

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Termination

End of translation when a stop codon is reached

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Release factors

Proteins that trigger ribosome disassembly at stop codons

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Reverse transcriptase

Enzyme in retroviruses that makes DNA from RNA

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Regulatory proteins

Proteins that control gene expression by binding to specific nucleotide sequences

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Regulatory sequences

Nucleotide sequences where regulatory proteins bind to control gene expression

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Mutations

Changes in the genome that can impact gene expression

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Operon

Cluster of genes with a common function controlled by a common promoter

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Operator

Noncoding sequence binding repressor proteins to regulate transcription

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Structural genes

Coding sequences containing genetic information for operon function

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Inducible operons

Operons activated by inducer molecules to perform specific functions

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Repressible operons

Operons that are normally active but can be inhibited by excess product

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Catabolic function

Operons involved in breaking down molecules for energy

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Corepressor

Molecule that binds to a repressor to inhibit transcription

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Cyclic AMP (cAMP)

Molecule whose interaction with CAP can upregulate gene expression

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Catabolite activator protein (CAP)

Protein that binds cAMP to stimulate transcription near the promoter

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trp repressor protein

Protein that binds to tryptophan before binding to the operator

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tryptophan

Amino acid that binds to trp repressor

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tor

RNA polymerase that transcribes the operon

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corepressor

Molecule that binds to repressor

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Promoters (Eukaryotes)

Binding sites for RNA polymerase in eukaryotic gene regulation

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Enhancers

Regulatory switches where activator proteins bind in eukaryotes

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Silencers

Regulatory switches where repressor proteins bind in eukaryotes

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Epigenetic changes

Reversible modifications to DNA affecting gene expression in eukaryotes

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Hox genes

Family of genes that code for transcription factors

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Antennapedia

Hox gene controlling leg formation in Drosophila

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Phenotype

Observable traits resulting from gene expression

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Natural selection

Process where beneficial traits are favored for survival

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Aneuploidy

Condition with an incorrect number of chromosomes

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Klinefelter syndrome

Genetic disorder with XXY sex chromosomes

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Down syndrome

Genetic disorder with an extra copy of chromosome 21

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Polyploids

Organisms with an entire extra set of chromosomes

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Horizontal transmission

Transfer of genetic information between same-generation members

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Transformation

Uptake of foreign DNA by a cell

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Transduction

DNA transmission between organisms by viruses

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Conjugation

DNA transmission through cell-to-cell contact

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Transposition

Movement of DNA between or within chromosomes

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Bacterial Transformation

Introduction of foreign DNA into bacterial cells

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Recombinant DNA

DNA recombined from different source organisms

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Restriction Endonucleases

Enzymes cutting DNA at specific sequences

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DNA Ligases

Enzymes connecting DNA fragments

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Recombinant Plasmids

Plasmids with selectable markers and desired gene

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Gel Electrophoresis

Separates DNA fragments by size and charge

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Agarose Gel

Matrix for DNA migration in gel electrophoresis

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Polymerase Chain Reaction (PCR)

Amplifies specific DNA fragments

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Denaturing

Separates DNA strands in PCR

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Annealing

Hydrogen bonding of primers in PCR

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Extension

DNA polymerase adds nucleotides in PCR

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CRISPR-Cas9

System editing DNA sequences using guide RNA

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Knockout

Disabling a gene's function using CRISPR

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DNA Sequencing

Determining the order of nucleotides in DNA

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Bacteriophages

Viruses infecting bacteria

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Guide RNA

Directs Cas9 to cut at specific DNA sequences

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