Bronchoalveolar Lavage (BAL)

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This is a method for obtaining cellular, immunologic, and microbiologic information from the lower respiratory tract

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1

This is a method for obtaining cellular, immunologic, and microbiologic information from the lower respiratory tract

Bronchoalveolar Lavage

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2

During bronchoscopy, a fiber-optic bronchoscope is guided into a selected bronchopulmonary segment, usually the?

Right middle or lingular lobe

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3

Sterile saline volume should be?

Between 100 and 300 mL

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4

Aliquots volume should be?

20 to 50 mL

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5

Which part of the aliquot should be discarded?

First aliquot

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6

Which part of the aliquot should be sent individually for analysis/pooled for further analysis

Remaining aliquots

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7

The desired fluid volume for analysis is?

10 to 20 mL

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8

Minimal fluid volume for analysis should be?

5 mL

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9

Specimens should be kept at a __ during transport to the laboratory and process them __

Room temperature; immediately

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10

When should you put your specimens on ice during transportation?

Delayed for longer than 30 minutes

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11

Specimens that will not be analyzed immediately should be?

Centrifuged

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12

Specimens are unacceptable for testing after?

24 hours

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13

Cell counts should be performed within?

1 hour or are stable for up to 3 hours

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14

Samples can be filtered with the use of?

Loose gauze

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15

Loose gauze is used to filter out what?

Mucus, phlegm, and dust

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16

A macroscopic observation is recorded describing the __ and __ of the specimen

Color and clarity

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17

BAL fluid color can be?

Clear, milky white, light brown-beige, and red

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18

____ is the result of an older hemorrhagic syndrome and would be evaluated for intracellular iron content

Orange-red BAL fluid

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19

This indicates an accumulation of phospholipid-protein complexes derived from pulmonary surfactant in the lung alveoli and strongly suggests pulmonary alveolar proteinosis

Milky or light brown-beige

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20

The BAL fluid should be centrifuged if it looks?

Milky

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21

In what degrees should the specimen be put when delayed

4 degrees celsius

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22

Which are performed on BAL and is also diluted to facilitate counting?

WBC and RBC

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23

WBC and RBC is diluted using a?

Hemocytometer

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24

Cell viability can be determined by adding?

Trypan blue

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25

If a hemocytometer is used, WBC counts may be diluted using the ____ to facilitate counting

BMP LeukoChek system

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26

A BMP LeukoChek system is available with a 1 to 100 dilution of __ to lyse the RBCs

Ammonium oxalate

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27

RBC count is diluted with _____ using an ______

Isotonic saline; MLA pipette

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28

For leukocytes, differential slides are prepared by?

Cytocentrifugation

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29
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30

This is the cell that is most frequently seen

Macrophages

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31

Macrophages contains a variety of?

Phagocytized materials

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32

Macrophage ranges from?

56% to 80%

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33

Lymphocytes ranges from?

1% to 15%

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34

Increased lymphocytes suggests?

Interstitial lung disease, drug reactions, pulmonary lymphoma, and non bacterial infection

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35

Lymphocyte with a differential count equal or greater than 25% suggests

Granulomatous lung disease

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36

Lymphocyte with a differential count greater than 50% suggests

Hypersensitivity pneumonitis or non specific interstitial pneumonia

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37

This leukocyte further defines the disease process

Ratio of CD4 to CD8 lymphocytes

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38

Elevated CD4/CD8 indicates?

Sarcoidosis or connective tissue disorders

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39

Normal CD4/CD8 indicates?

Tuberculosis or malignancies

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40

Low CD4/CD8 indicates?

Hypersensitivity pneumonitis, silicosis, drug induced disease, or HIV infection

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41

Immunologic analysis is performed by?

Flow cytometry

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42

What is the primary granulocyte that is seen in BAL

Neutrophils

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43

Normal value of neutrophils

Less than 3%

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44

Neutrophils count equal to or greater than 50% strongly suggests

Acute lung injury, aspiration pneumonia, or suppurative infection

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45

Eosinophil with a differential count greater than or equal to 25%

Eosinophilic lung disease

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46

CASE STUDY. When you get the result of a mast cell differentiation greater than 1%, a lymphocyte counts greater than 50% as well as a neutrophil count greater than 3% strongly suggests that this patient has?

Hypersensitivity pneumonitis

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47

Presence of erythrocytes indicates?

Acute Alveolar hemorrhage

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48

Alveolar hemorrhage has occured within the past 48 hours indicates a presence of?

Phagocytosed erythrocytes

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49

Presence of Hemosiderin-laden macrophages indicates?

Alveolar hemorrhage older than 48 hours

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50

In lavage specimen, epithelial cells normally range from?

4% to 17%

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