Studied by 50 peopleTags & Description
Precipitation and Coagulation
What are the two tests to isolate proteins from samples?
Native Confirmation
Proteins assume a specific three-dimensional structure called __
Globular (spherical)
The native confirmation of Albumin is __
Fibrous (elongated)
The native confirmation of Gelatin is __
Denaturation
This is the change in protein native conformation; the disruption and unfolding of protein
Reversible and Irreversible
What are the two types of denaturation?
Coagulation
This refers to the clumping of particles together in a solution
Alcohol and Heat
What are the two causes of coagulation?
Coagulum
What is the result of coagulation?
Solids clumping together
What is the appearance/result of coagulation?
Ethanol and Heating
These materials were used to observe the coagulation of egg albumin
Precipitation
The refers to the sticking of molecules together to form a solid that will come out from the solution
Heavy metals, Strong mineral acids, and Alkaloidal reagents
What are the causes of precipitation in egg albumin?
Coagulation by Heat/Alcohol
This result is due to?
Precipitation by Heavy Metal Salts
This is a result due to?
Silver Nitrate (AGNO3)
The reagent used for this test is?
Precipitation by Heavy Metal Salts
This is a result of?
Cupric Sulfate (CuSO4)
The reagent used for this test is?
Precipitation by Strong Mineral Acids
This is a result of?
Heller’s Test
The test for this reaction is called?
Nitric Acid (HNO3)
What reagent was used for this test?
Precipitation by Strong Mineral Acids
This is a result of?
Robert’s Test
The test for this reaction is called?
Conc. Nitric Acid + Sat. Magnesium Sulfate (HNO3 + MgSO4)
What reagent was used for this test?
Precipitation by Alkaloidal Reagents
This is a result of?
Picric Acid
The reagent used for this test is?
Precipitation by Alkaloidal Reagents
This is a result of?
Tannic Acid
The reagent used for this test is?
Color Reaction
This is a chemical reaction that is used to transform colorless chemical compounds into colored derivatives which can be detected visually.
Biuret Test
This is a test for peptide bonds.
Positive
Biuret Test: Is this a positive or negative result?
Negative
Biuret Test: Is this a positive or negative result?
Ninhydrin Test
This is a test for proteins based on free alpha amino groups.
Positive
Ninhydrin Test: Is this a positive or negative result?
Negative
Ninhydrin Test: Is this a positive or negative result?
Xanthroproteic Test
This is a test for phenyl-containing amino acids.
Phenyl-containing Amino Acids
What does this test indicate?
Adamkiewicz Test
This is a test for the presence of tryptophan.
Tryptophan
What does this test indicate?
Reduced Sulfur Test
This is a test for the presence of cysteine.
Cysteine
What does this test indicate?
Molisch Test
This is a test for the presence of carbohydrates in solution.
Carbohydrates
What does this test indicate?
Diluted Sodium Hydroxide (NaOH)
Among these reagents, which is yeast RNA fully soluble with?
Hot Water
Among these reagents, which is yeast RNA slightly soluble with?
Cold Water, 95% Ethanol, Hydrochloric Acid (HCl)
Among these reagents, which is yeast RNA insoluble with?
Sulfuric Acid (H2SO4)
What solution is needed for yeast RNA hydrolysis?
Test for Purine
Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?
Ammoniacal Silver Nitrate (Ammoniacal AgNO3) + Concentrated Ammonium Hydroxide (NH4OH)
Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?
Test for Pentose
Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?
Molisch Reagent + Concentrated Sulfuric Acid (H2SO4)
Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?
Test for Phosphate
Tests for the Chemical Component of Nucleic Acids: Based on the result, which test is this?
Ammonium Hydroxide (NH4OH) + Nitric Acid (HNO3) + Ammonium Molybdate ((NH4)2Mo7O24)
Tests for the Chemical Component of Nucleic Acids: What reagent(s) was/were needed to fulfill this test?
Note
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