5’ Cap
A modified form of guanine nucleotide added onto the 5′ end of a pre-mRNA molecule.
Alternative RNA Splicing
A type of eukaryotic gene regulation at the RNA-processing level in which different mRNA molecules are produced from the same primary transcript, depending on which RNA segments are treated as exons and which as introns.
Coding Strand
Nontemplate strand of DNA, which has the same sequence as the mRNA except it has thymine (T) instead of uracil (U).
Codon
A three-nucleotide sequence of DNA or mRNA that specifies a particular amino acid or termination signal; the basic unit of the genetic code.
Domain
(1) A taxonomic category above the kingdom level. The three domains are Archaea, Bacteria, and Eukarya. (2) A discrete structural and functional region of a protein.
Exon
A sequence within a primary transcript that remains in the RNA after RNA processing; also refers to the region of DNA from which this sequence was transcribed.
Intron
A noncoding, intervening sequence within a primary transcript that is removed from the transcript during RNA processing; also refers to the region of DNA from which this sequence was transcribed.
Messenger RNA
A type of RNA, synthesized using a DNA template, that attaches to ribosomes in the cytoplasm and specifies the primary structure of a protein. (In eukaryotes, the primary RNA transcript must undergo RNA processing to become mRNA.)
Primary Transcript
An initial RNA transcript from any gene; also called pre-mRNA when transcribed from a protein-coding gene.
RNA Polymerase
An enzyme that links ribonucleotides into a growing RNA chain during transcription, based on complementary binding to nucleotides on a DNA template strand.
RNA Processing
Modification of RNA primary transcripts, including splicing out of introns, joining together of exons, and alteration of the 5′ and 3′ ends.
RNA Splicing
After synthesis of a eukaryotic primary RNA transcript, the removal of portions of the transcript (introns) that will not be included in the mRNA and the joining together of the remaining portions (exons).
Signal Peptide
A sequence of about 20 amino acids at or near the leading (amino) end of a polypeptide that targets it to the endoplasmic reticulum or other organelles in a eukaryotic cell.
Signal-Recognition Particle
A protein-RNA complex that recognizes a signal peptide as it emerges from a ribosome and helps direct the ribosome to the endoplasmic reticulum (ER) by binding to a receptor protein on the ER.
Spliceosome
A large complex made up of proteins and RNA molecules that splices RNA by interacting with the ends of an RNA intron, releasing the intron and joining the two adjacent exons.
Template Strand
The DNA strand that provides the pattern, or template, for ordering, by complementary base pairing, the sequence of nucleotides in an RNA transcript.
Transcription
The synthesis of RNA using a DNA template.
Transcription Factor
A regulatory protein that binds to DNA and affects transcription of specific genes.
Transcription Initiation Complex
The completed assembly of transcription factors and RNA polymerase bound to a promoter.