Exam 3: Biology

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Enzymes

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39 Terms

1

Enzymes

-biological catalysts that speed up chemical reactions -require activation energy -only facilitates 1 type of reaction

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2

Active Site

interacts with substrates; have chemical charges or not

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3

Substrate

-a molecule that interacts with an enzyme -physical and chemical properties have to be compatible with the active site

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4

Allosteric site

region on enzyme where noncompetitive inhibitors can bind too

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5

Competitive Inhibitor

-bind to active sites -competes with normal substrates for active site -binding can be irreversible: enzyme function prevented -binding can be reversible: function is regained

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6

Noncompetitive Inhibitor

-bind's to enzyme's allosteric site -changes enzymes activity and shape: causes reaction rate to decrease

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7

Product

-what is produced by the chemical reaction of an enzyme -differs between each enzyme

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8

Denaturing of an enzyme

  • changes from environmental temperature or pH level change -change from competitive and noncompetitive inhibitors

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9

Temperature

increase of _________ outside of optimum range results in enzyme denaturation

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10

pH Level

increase or decrease of _________ outside of optimum range results in denaturation

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11

Photosynthesis

biological process that captures energy from the sun and produces sugars

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12

6CO2 + 6H2O + Photons >>>> C6H12O6 +6O2

photosynthesis formula

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13

Palisade Mesophyll

type of leaf cell that contains chloroplasts

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14

Light Dependent Reaction

step 1 of photosynthesis

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15
  1. Photo Excitation

light photon strikes chlorophyll in PSII, which energizes the e- ; e- leaves

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16
  1. Electron Transport Chain

e- jumps down the ETC, losing energy as it goes. Some released energy moves hydrogen across the membrane from the stroma to the thylakoid. e- loses energy and tops in PSI

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17
  1. Electron Transport Chain

Photon Excitation occurs in PSI, which energizes the e-. The e- jumps down ETC and into the Stroma

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18
  1. Reduction NADP+ to NADPH

the e- that enters the stroma meets the NADP+ and changes it into NADPH

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19
  1. Photolysis

sunlight breaks down H2O. The H2 is added into the thylakoid. O2 is the byproduct and is released by the plant. e- is stripped from H and replaces the e- in PSII

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20
  1. Chemiosmosis

H+ gradient is established, ATP synthase will push H+ ions to reduce ADP to ATP

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21
  1. Reduction ADP to ATP

ATP synthase will use the power from H+ ion to reduce ADP to ATP

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22

NADPH and ATP

molecules created for the Calvin Cycle

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23

Hydrolysis Reaction

the hydrogen molecules from the splitting of water are released into the thylakoid space and are used to create H+ ion gradient

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24

Location of Photosynthesis

in chloroplasts; light reactions occur in the thylakoid with the help of the stroma

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25

Calvin Cycle

-Step 2 of Photosynthesis -uses NADPH and ATP from the light dependent reaction

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26

Rubisco

the enzyme used in the Calvin Cycle to bond CO2 and RuBP

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27
  1. Carbon Fixation

CO2 binds to RuBP sugar with the help of the Rubisco enzyme

  • 6-carbon molecules split into 3-carbon molecules

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28
  1. Activation and Reduction

3-PGA is converted into G3P. ATP and NADPH are added -ADP and NADP+ returns to the thylakoid

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29
  1. Replacement of RuBP

1 G3P is kept while 5 G3P are used to make RuBP with ATP

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30

2 times

the Calvin Cycle must be repeated _ _____ in order to make 1 glucose

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31

Stroma

location of the Calvin Cycle

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32

Cellular Respiration

involves the capture of energy from macromolecules, which then releases chemical energy through the break-down of glucose and creates ATP

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33

Anaerobic Respiration

Production of ATP when O2 is not present -fermentation -allows glycolysis to proceed in the absence of O2

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34

Lactic Acid

byproduct of fermentation

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35

Aerobic Respiration

the production of ATP -occurs in the linking reaction, Krebs Cycle, and electron transport chain

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36

Linking reaction

-requires O2 -occurs in the intermembrane of the mitochondria -makes Acetyl CoA from pyruvates and NAD+

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37

Krebs Cycle (Citric Acid Cycle)

-requires O2 -occurs in the matrix of mitochondria -uses Acetyl CoA + NAD+ + FAD + ADP + to make NADH + FADH2 + ATP +CO2

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38

Electron Transport Chain

-requires O2 -occurs in the inner mitochondrial membrane (matrix and inner membrane)

  • uses NADH and FADH2 to energize electrons. The e- goes down the ETC, releasing hydrogens. Through Chemiosmosis, the hydrogens bond with O2 to form water and 36 ATP are created through ATP Synthase

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39

ATP Hydrolysis

-the _________ of ATP molecules results in a net output of energy -splitting bonds of ATP, creating energy for the cell

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