Radioactive amino acids
________ can be used for pulse labeling a cohort of proteins that are newly made in the ER.
Assays
________ that follow protein trafficking through a secretory pathway need to label a cohort and identify where the labeled proteins are located.
Nerve
________ impulses transmission at chemical synapses depend on the exocytosis of neurotransmitter filled synaptic vesicles found in cells along with those empty vesicles regenerating.
Lysosomes
________ end incorporated into vesicles inside the endosome if they endocytose proteins meant for degradation.
Endocytic budding
________ rapidly regenerates synaptic vesicles which are coated in clathrin and this coating coming off helps the vesicles refill their neurotransmitter and move to the active zone, where they are once again put through docking and fusion.
Yeast
________ contains many of the components needed for intracellular protein trafficking.
cis Golgi
The ________ contains the membrane proteins needed for the formation of COPII vesicles, and COPI vesicles can retrieve them.
Polymerization
________ is controlled by GTP binding proteins which are a part of the GTPase superfamily.
Vesicles
________ from the trans- Golgi network along with endocytic vesicles have a coat of AP (adapter protein) complexes.
Internalization
________ can occur with some extracellular ligands which bind to cell surface receptors, and the clathrin- coated vesicles that the receptors are ________ into also contain AP2 complexes.
endocytic pathway
A(n) ________ helps to transport Iron into the cell.
COPII
Proteins are transported through the rough ER with the help of ________, and COPI vesicles transport those proteins in the opposite direction.
luminal proteins
Sorting signals found in the membrane interact with coat proteins along with ________ of the donor organelles during vesicle budding, during cargo proteins into vesicles.
Lysosomes
________ are degraded once they are delivered through the endocytic pathway.
cis Golgi
The ________ modifies soluble enzymes that are meant to go into the lysosomes.