Gene Teraphy & Gene Slicing

Replacing an abnormal gene with a healthy copy of the gene

Inactivating an abnormal gene that is functioning improperly

Process by which the instructions in our DNA are converted into a functional product, such as a protein.

Gene is expressed when there is:

is used to identify or determine gene expression and also regulation.

If all genes are turned ON, there is a possibility for ________ cell.

What is enzymes is off in the Muscle

What is enzymes is off in the pancreatic islet

measure the expression of levels of large numbers of genes simultaneously or to genotype multiple regions of a genome.

sample to get a suspected sample of healthy and cancer cells

Acts as “wire or adaptor” to the mRNA

An enzyme which will synthesize a strand of complementary DNA (cDNA)

color that indicates healthy cells

color that indicates cancerous cells

color that indicates both healthy and cancerous cells

Also known as recombinant DNA technology

Comprises multiple techniques for the intentional manipulation of genetic material to alter, repair, or enhance form or function

Experimental technique that uses genes to treat or prevent disease

year when Genetic Engineering was first presented at Sixth International Congress of Genetics

year and who suggested that genes could be transferred within nucleic acids

first use the term gene therapy

year that set the basis of molecular genetics and gene transfer

at what year that The first deliberate transfer of foreign genes into human recipients with a therapeutic purpose, and was performed by ___________

in july 1980, who headed another highly controversial human trial designed to treat B-thalassemia

in this year, Enzyme-producing-gene-corrected cells were further theorized as viable approach for treating Lesch-Nyhan disease

Will not be inherited in later generations

Example: genes are introduced to egg cell or sperm cell

introduction of genes into bone marrow cells, blood cells,

Can be Ex vivo or in vivo

Non-viral delivery system

Viral delivery system

Viruses currently being used for HCC gene therapy

Cells modified in vitro Modified cell – place inside the sterile petri dish

Vehicle for gene delivery

TYPES OF VIRAL VECTORS

It is the simplest non-viral method

Clinical trials carried out intramuscular injection of the _______ plasmid have occurred with some successes.

Used of the synthetic oligonucleotides and the gene therapy can activate genes involved disease process

Improve the delivery of new DNA into the cell.

It generally describe the “switching off’ of a gene by a mechanism other than genetic modification

same as gene knock down but is totally different from gene knock out

Technique that aims to reduce or eliminate the production of a protein to its corresponding gene

in 1990, who deepen the pigmentation in petunias Introduction of transgenes homologous to endogenous genes often resulted in plants with both gene suppressed called – co suppression

In this year, Guo and Kemphues - Injection of either antisense or sense RNA in the germline of C.elegans was equally effective at silencing at homologous target genes

in 1998, this two scientist provide an extension of the experiment of Guo and Kemphues

Year when Synthetic exogenous siRNA to induce sequence-specific gene knockdown in mammalian cells in vitro

year when the First description of dsRNA as trigger for gene specific silencing and term as RNAi

in year 2002, first in vitro gene slicing with RNAi is done in what animal?

Year when a Systemic administration of siRNA in non-human primates. it is also the same year when Andry Fire and Craig awarded the Nobel Prize for discovery of RNAi

Proof of concept for use of an RNAi therapeutic in humans was provided in this year

This year provided Explosive growth for basic research and therapeutic intervention in various diseases