Cell Bio- Exam 1 

________- glucose transporter expressed on plasma membrane of RBCs and endothelium or BB barrier, can pump one glucose at a time.

________ inserts into membrane and polar group will extend beyond membrane.

________ clamping- can use small needle to clamp onto membrane and suck part of the membrane with the channel in, we can measure current flow with this technique.

________ seq- stops translocation into cytosol, translocation into lumen commences and transcription is cont.

________- conjugation happens on c terminus side.

________:: sphingolipid, cholesterol and certain proteins make up.

________- small nutrients taken up by the cell, fluid droplets.

________ (ex to c) and floppase (c to ex) can aid in spontaneous flipping in the membrane, use ATP.

________- has sugar and phosphate, conjugate to membrane on c terminus, face towards exoplasmic surface.

________: may not accurately represent the og cells in tissue.

________ for biochemical activities- certain proteins can receive signals on membrane.

________- water transport pore, transports water across membrane for water balance, arrangement inside porin prevents protons from entering.

Fix:: using ________ to kill /section the cell, partially permeabilizes cells.

________- has double layer membrane, used to deliver drugs.

________ and PI carry neg charges→ help determine charge of membrane.

________ on the surface by lipid is conjugated to ________ and lipid is in the bilayer.

________- reads mito targeting signal, delivers to translocon.

No ________ is bound to protein, peptide chain goes through translocon.

________ binds to GTP which is bound to the translocon.

Catalyzes the ________ and rearragement of disulfide bonds (key for proper folding, function, and stability)

________:: flip flop from one face to another, hard bc head group does not like to cross hydrophobic environment.

________ is receptor, binds to Pex14, transfers to Pex12 /2, then ________ releases.

________:: mRNA molecule to which ribosomes are attached and engaged in protein synthesis.

________ formed to localize biochemical reactions.

________:: only can recognize a single epitope, used for covid treatment.

________ hydrolyzes to transfer c term into ER mem.

10-30 micro m in size

lineage of cells originating from one initial primary cell culture

may not accurately represent the og cells in tissue

only can recognize a single epitope , used for covid treatment

inject antigen into animal, animal generates b cells to recognize antigen, myeloma cells fuse with b cells, transfer to to selective medium and find fused cells

glass lens close to sample

close to eye

using diff chemicals to kill/section the cell, partially permeabilizes cells

cut into small pieces to look directly at underneath the microscope

stain nucleus, base, purple

stains cytoplasm pink, acid

absorb light at shorter wavelength (excitation), emit light at longer wavelength (emission)

certain proteins can receive signals on membrane

certain mem proteins can transport things

have two diff properties, hydrophobic and hydrophilic

double bonds

no double bonds

has double layer membrane, used to deliver drugs

one layer membrane

flip flop from one face to another, hard bc head group does not like to cross hydrophobic environment

move across own face

turn around

sphingolipid, cholesterol and certain proteins make up

faces to cytosol in cell/ matrix

faces exterior, or intermembrane space

long hydrophobic and hydrophilic tails, can interact with a protein to keep it dissolved into the solution that it is in (SDS, Triton)

use fatty acids and conjugation on n terminal side

conjugation happens on c terminus side

has sugar and phosphate, conjugate to membrane on c terminus, face towards exoplasmic surface

contains conc gradient and membrane potential (cyto more neg and exoplasmic side more positive)

glucose transporter expressed on plasma membrane of RBCs and endothelium or BB barrier, can pump one glucose at a time

water transport pore, transports water across membrane for water balance, arrangement inside porin prevents protons from entering

absence of it leads to diabetes, excretion of large vols of dilute urine

inside cell is negative, charge accumulates on sides

can use small needle to clamp onto membrane and suck part of the membrane with the channel in, we can measure current flow with this technique

HCO3 in, Cl out, HB in so that CO2 can leave

small nutrients taken up by the cell, fluid droplets

secretion of obj out of cell

uses ATP to move Na and K from low to high conc

uses Na and mem potential to pump glucose and Na from low to high conc

glucose uniporter

attached to cytoplasmic face of ER, synthesize secretory proteins

free in cytosol, synthesizes non secretory proteins

mRNA molecule to which ribosomes are attached and engaged in protein synthesis

plasma membrane, lysosome, endosome

translocation stops, translating continues

stops translocation into cytosol, translocation into lumen commences and transcription is cont

n term faces cytosol

n term faces lumen

reads mito targeting signal, delivers to translocon

associates with import receptor, delivers protein to 2nd translocon

aligned with TOM

aids in net translocation

spans both bilayers, huge

occurs via diffusion