what is bulk flow?
fluids flow down pressure gradients (high → low pressure), how larger animals move fluid through their bodies
what is the law of bulk flow?
Q = ∆P/R
what is resistance?
due to friction, opposes fluid movement; R = 8ηL/πr4
what is poiseuille’s equation?
Q = ∆Pπr4 / 8ηL
what do circulatory systems transport throughout the body?
oxygen, carbon dioxide, nutrients, waste products, immune cells, signaling molecules
what is ohm’s law?
I = V / R
how does a circulatory system move fluids?
by increasing pressure of the fluid in one part of the body
how is resistance related to vessel radius?
as radius increases, resistance decreases, and vice versa
what are assumptions for poiseuille’s equation?
unbranched and rigid tubes
uniform and simple flow
steady flow
explain resistors in series
total R is the sum of each R value
each section between each resistor in a blood vessel has its own pressure
explain resistors in parallel
to find the total R, add the inverse of each R value together (1/R)
pressure before branching is higher than pressure after branching
what is important about flow in a closed circuit?
flow is uniform at all points within a closed circuit
what is the relationship between flow, area, and velocity of flow?
V = Q / A
velocity is NOT uniform at all points within the circuit
where is blood velocity lowest in the human body?
at the circulatory system’s capillary beds, where total cross-sectional area is highest
how many circuits and chambers does a fish have?
1 and 2
how many circuits and chambers does a turtle/lizard have?
2 and 5
how many circuits and chambers do mammals have?
2 and 4
what are the two circuits in the mammalian circulatory system?
pulmonary (heart-lungs) and systemic (heart-body)
describe blood flow in and out of the heart
deoxygenated blood enters the right atrium via the vena cava, then goes into the right ventricle and is pumped to the lungs via the pulmonary artery
oxygenated blood enters from the lungs to the left atrium via the pulmonary veins, then goes into the left ventricle and is pumped to the body via the aorta
describe the names of blood vessels based on direction of flow
arteries carry blood from the heart
arterioles are smaller branches of an artery
veins carry blood towards the heart
venules are smaller branches that combine to make up a vein
what is the structure of a vertebrate heart wall?
largely made up of myocardium, with endothelium on the inside and pericardium on the outside (with pericardial fluid in the pericardial cavity)
what is the function of the pericardium?
this multi-layered section of the heart protects it from friction while it’s beating
how is the myocardium of a fish different than the myocardium of a mammal?
fish have a spongy myocardium, which allows blood from heart chambers to get to the myocardium and deliver nutrients
what is diastole?
relaxation of the heart, negative pressure, blood flows in
what is systole?
contraction of the heart, positive pressure, blood flows out
explain blood flow in and out of the heart in terms of systole/diastole of the atria and ventricles
ventricular diastole - atria pressure > ventricular pressure, and av values open. during total relaxation, 70% of the blood flows from the atria to the ventricles, called passive filling
atrial systole - the atria contract, forcing the remaining 30% into the ventricles
ventricular systole - the atria relax while the ventricles contract (and av valves close), called isovolumetric contraction (increase in pressure until the semilunar valves open)
ventricular systole - when ventricular pressure is high enough, semilunar valves open and ventricle contraction causes ejection of blood into arteries
ventricular diastole - the ventricles relax, called isovolumetric relaxation (semilunar valves are closed while ventricular pressure decreases)
what is an important quality of heart valves?
they are passive, meaning no muscles or ATP are used, they only respond to pressure gradients
what are av valves?
atrial → ventricular, right - tricuspid, left - bicuspid/mitral, weak enough to open with pressure but not backwards (prolapse), chordae tendinae and papillary muscles prevent prolapse
what are semilunar valves?
ventricular → out, pulmonary valve (to lung) and aortic value (to body), weak enough to open from ventricular contraction but withstand return pressure
describe the pathway of conducting myocardium
the SA node sends out an action potential and it goes to the AV node via junctional fibers
the signal travels from the AV node to the AV bundle, aka bundle of His, and then splits down the left and right bundle branches
the bundle branches split off into purkinje fibers and the signal travels into the walls of the ventricles, causing them to contract
what is the pacemaker potential of the SA node?
it has high Na+ permeability and low K+ permeability, which causes slow, continuous depolarization
this pacemaker potential causes the SA node to reach its threshold and trigger an action potential
after the SA node repolarizes, the pacemaker potential occurs again
what allows the pacemaker potential to occur?
the “funny current” through the funny channel — a non-selective cation channel that opens at hyperpolarized voltages and closes at depolarization, mostly Na+
the SA node essentially has an intrinsic action potential frequency, slowly getting to threshold, then action potential, then repeat
what is the cause of the plateau in the cardiac action potential?
a special kind of K+ channel called a transient channel, which is open only for a short time and causes a small amount of repolarization before closing again
Ca2+ is flowing into the cell at the same time, causing the stable plateau
explain an EKG wave
P-wave shows the depolarization of atria (contraction)
QRS complex shows depolarization of ventricles (contraction) and repolarization of atria (relaxation)
T-wave shows repolarization of ventricles (relaxation)
how do the parasympathetic and sympathetic branches of the autonomic nervous system reciprocally control heart rate?
they control the pacemaker potential in the sa node
what are some ways to change heart rate?
controlled by the sympathetic and parasympathetic nervous system
sympathetic system creates faster depolarization, limits the funny current, while the parasympathetic system creates longer depolarization, increasing the funny current
describe the parasympathetic pathway of the SA node
acetylcholine activates both muscarinic receptors of autorhythmic cells
K+ leaves, Ca2+ stops entering, cell hyperpolarizes
more time needed to depolarize, HR decreases
describe the sympathetic pathway of the SA node
norepinephrine and epinephrine activate beta receptors of autorhythmic cells
adenylate cyclase converts ATP to cAMP which contributes to ions entering cell
Na+ and Ca2+ both enter
depolarization increases, HR increases
what is the driving force of blood flow throughout the circulatory system?
the pressure caused by ventricular ejection
what is cardiac output?
CO = HR x SV
how does the sympathetic nervous system modulate the force of heart contractions (stroke volume)?
norepinephrine or epinephrine binds and changes the shape of beta receptors
adenylate cyclase converts ATP to cAMP
cAMP activates protein kinase A
protein kinase phosphorylates L-type Ca2+ channels so Ca2+ enters the cell, stimulating contraction
protein kinase phosphorylates Ca2+ channels on SR, Ca2+ moves to cytoplasm and stimulates contraction
protein kinase phosphorylates myosin, stimulating contraction
protein kinase phosphorylates sarcoplasmic Ca2+ ATPase, speeding removal of Ca2+ from cytoplasm, decreasing relaxation time
increasing contraction force increases stroke volume, which increases cardiac output
what is systolic pressure?
highest arterial blood pressure, from ventricular contraction
what is diastolic pressure?
lowest arterial blood pressure, from ventricular relaxation?
what is mean arterial pressure (map)?
average blood pressure in arteries, 2/3 DP + 1/3 SP, MAP = CO x TPR
what is the order from highest to lowest blood pressure?
left ventricle → arteries → arterioles → capillaries → venules → veins
how do baroreceptors monitor blood pressure?
they detect a rise in blood pressure, signal to the cardiovascular control center of the medulla, and enact a negative feedback loop
what is water necessary for?
enzymatic reactions, gas exchange, etc.
describe the typical ion concentrations in mammalian blood
300 mOsM total, most of it is Na+ and Cl-, the rest are small amounts of K+, Ca2+, Mg2+, and (SO4)2-
describe the typical ion concentrations in seawater
1000 mOsM total, most of it is Na+ and Cl-, the rest are small amounts of K+, Ca2+, Mg2+, and (SO4)2-
describe the typical ion concentrations in freshwater
< 5 mOsM total, most comes from Ca2+, (SO4)2-, Na+, and Cl-, with slightly smaller amounts of Mg2+ and K+
what is the difference between tonicity and osmolarity?
“tonic” refers to one solute, while “osmotic” refers to all solutes
what is an isosmotic solution?
concentration of solutes outside cell = concentration of solutes inside cell, net flow is 0
what is a hyperosmotic solution?
concentration of solutes outside cell > concentration of solutes inside cell, water leaves cell (shrinks)
what is a hyposmotic solution?
concentration of solutes outside cell < concentration of solutes inside cell, water enters cell (swells)
how do marine animals deal with ionic and osmotic challenges?
they gain salts and lose water
how do freshwater animals deal with ionic and osmotic challenges?
they gain water and lose salts
how do terrestrial animals deal with ionic and osmotic challenges?
they lose water
how do animals that move between these environments deal with ionic and osmotic challenges?
they can alter their osmotic/salt homeostasis
what are osmoconformers?
typically invertebrate aquatic animals that change their body’s osmolarity to be similar to their environment
what are osmoregulators?
typically vertebrate aquatic animals that maintain constant osmolarity regardless of the environment
what are ionoconformers?
typically invertebrate animals that exert little control over ion profile within their extracellular space
what are ionoregulators?
typically vertebrate animals that control ion profile within their extracellular space
what is a perturbing solute?
it increases the Km (rate of reaction) within an organism, the most important ones being nitrogenous wastes or NaCl; too much can disrupt macromolecule function and kill
what is a compatible solute?
it has no effect on Km (rate of reaction) within an organism, examples being amino acids or methyl amine
what is a counteracting solute?
it’s deleterious on its own but can be used in combination to counteract the deleterious effects of another; ex: urea + THAMO has no effect as they have inverse effects on Km
what is a stenohaline organism?
they can tolerate only a narrow range of salinity
what is a euryhaline organism?
they can tolerate a wide range of salinity
what are four features of transport epithelial cells?
asymmetrical distribution of membrane transporters (solutes selectively transported)
cell interconnected to form impermeable sheets of tissue (little leakage in between)
high cell diversity within tissue
abundant mitochondria (large energy supply)
apical cell side vs basolateral cell side
the basolateral side is always connected towards the blood, while the apical cell is opposite and sometimes has cilia
describe transcellular transport
movement through the cell across membranes
describe paracellular transport
movement between cells, can be leaky or tight epithelia
list some types of transporters
Na+/K+ ATPase - with or against gradient
ion channels (Na+, Cl-, K+) - with gradient, no energy
electroneutral cotransporters - transfer ions with opposite charges to flow in same direction
electroneutral exchangers - transfer ions with same charge to flow in opposite directions
what is a problem saltwater fish face and how do they compensate?
they are always losing water and gaining salt, so they compensate by having extremely concentrated urine
what is a problem freshwater fish face and how do they compensate?
they are always gaining water and losing salt, so they compensate by having extremely dilute urine
which of the following would be describing the osmotic challenge of a tuna fish? (they live in the ocean)
they are hyperosmotic to their environment
they tend to make dilute urine
they will uptake salts and ions through their gills
they will take up water from their environment faster than they can remove it with their kidneys
they need to work to remove a lot of extra water from their blood
they will uptake salts and ions through their gills
what are fish gill lamellae composed of?
mitochondria-rich chloride cells and pavement (mitochondria-rich or mitochondria-poor) cells; transport is carried out by mitochondria-rich cells
what are diadromous fish?
fish that can migrate between saltwater and freshwater
what are catadromous fish?
fish that live in freshwater and migrate to saltwater to spawn
what are anadromous fish?
fish that live in saltwater and migrate to freshwater to spawn
what is common between terrestrial and marine animals regarding osmoregulation?
both lose water to their environment
how do we control water loss?
skin and hydrophobic layers
what terrestrial animals cover their external surfaces with mucus to reduce water loss?
amphibians
what terrestrial animals cover their external surfaces with cornified stratum corneum containing keratin and sebaceous tissues?
humans, amniotes (vertebrates that are exclusively land animals, unlike amphibians and fish)
what terrestrial animals cover their external surfaces with cuticles containing chitin?
arthropods (insects, crustaceans, etc.)
describe the basic structure of the skin
top layer is epidermis, bottom layer is dermis, separated by basement membrane
epidermis contains stratum corneum, corneocytes that maintain the stratum corneum, and keratinocytes that make keratin
the dermis has blood vessels and nerves
what are the three sources of water for terrestrial animals?
dietary water (from pre-formed water in plants and animals)
metabolic water (generated from final steps of cellular respiration)
drinking
what are the ways we lose water?
urine, evaporation, feces
why do we lose so much water, instead of recycling/maintaining it like a kangaroo rat?
nitrogenous wastes are toxic and need to be excreted
what are three forms of ammonia nitrogen that are excreted?
ammonia (ammonioteles)
uric acid (uricoteles)
urea (ureoteles)
what kind of animals are ammonioteles?
aquatic animals, because ammonia is water-soluble
what kind of animals are uricoteles?
terrestrial animals like birds, reptiles, and insects, uric acid isn’t very dangerous but it doesn’t dissolve in water
what kind of animals are ureoteles?
all mammals, some larval bony fish, lungfish
what are some advantages and disadvantages of ammonia excretion?
advantages: released by deamination of amino acids, requires little energy to produce (0-1 ATP)
disadvantages: highly toxic (used as cleaners), requires lots of water to store and excrete
what are some advantages and disadvantages of uric acid excretion?
advantages: few toxic effects, can be excreted in small volume of water
disadvantages: expensive to produce (6 ATP)
what are some advantages and disadvantages of urea excretion?
advantages: only slightly toxic, relatively energy inexpensive to produce (3 ATP)
disadvantages: it’s a perturbing solute
what strategy do we use to remove nitrogenous wastes?
production of hyperosmotic (concentrated) urine
what are six roles that vertebrate kidneys play in homeostasis?
ion balance
osmotic balance
blood pressure
pH balance
excretion of metabolic wastes and toxins
hormone production (renin-angiotensin system)
where is urine collected in the kidney before it goes to the bladder via the ureter?
minor and major calyx
what is the difference between urine in the bladder and urine in the renal pyramid?
urine in the bladder is the final urine, urine in the renal pyramids is primary urine
what is the renal pyramid divided into?
renal cortex and renal medulla