DNA & DNA replication

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nucleotide

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20 Terms

1

nucleotide

A building block of DNA, consisting of a five-carbon sugar covalently bonded to a nitrogenous base and a phosphate group.

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2

antiparallel

Strands of a DNA double helix have their nucleotides oriented in the opposite direction to one another.

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3

DNA replication

The process in which DNA makes a duplicate copy of itself.

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4

DNA gyrase

Enzyme that functions to reduce DNA supercoiling and relieve tension in DNA.

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5

DNA helicase

An enzyme that unwinds the DNA double helix by breaking H bonds during DNA replication.

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6

single-stranded binding proteins

Molecules that prevent the DNA strands from reannealing.

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7

DNA polymerase III

Enzyme that synthesizes DNA in the 5' to 3' direction.

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8

DNA polymerase I

Enzyme that removes the RNA primer and replaces the nucleotides with DNA.

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9

DNA polymerase II

Enzyme that proofreads the newly synthesized DNA.

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10

DNA ligase

Enzyme that joins Okazaki fragments.

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11

RNA primase

Enzyme that synthesizes the RNA primer.

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12

RNA primer

Short segment of RNA used to initiate synthesis of a new strand of DNA during replication.

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13

leading strand

A continuously synthesized DNA strand that elongates towards the replication fork.

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14

lagging strand

A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork.

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15

replication fork

A Y-shaped region on a replicating DNA molecule where new strands are growing.

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16

replication bubble

Segment of a DNA molecule that is unwinding and undergoing replication.

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17

complementary base pairing

Hydrogen bonding between specific pyrimidines and purines (adenine & thymine; cytosine & guanine)

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18

5'

End of a DNA molecule with a free phosphate group.

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19

3'

End of a DNA molecule with a free hydroxyl group.

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20

double helix

The shape of DNA; referring to its two adjacent polynucleotide strands wound into a spiral shape.

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