Chapter 19: Variable Number Tandem Repeat Profiling

the first historical method used in forensic DNA testing.

It utilizes restriction endonucleases that recognize and cleave specific sites along the DNA sequence.

These are enzymes that cleave the phosphodiester bond of DNA at or near specific recognition nucleotide sequences known as restriction sites.

It often has a specific palindromic recognition sequence, that is, a segment of double-stranded DNA in which the nucleotide’s sequence is identical with an inverted sequence in the complementary strand.

It is also known as Southern blotting.

This method can be used to transfer DNA from an agarose gel to a solid matrix so that it can be detected with a hybridization probe.

Southern Transfer is named after?

It can detect multiple VNTR loci simultaneously.

It generates a simple pattern called a DNA profile, consisting of one band for a homozygote and two bands for a heterozygote per locus.

It is also known as Southern blotting.

A range of DNA fragments that differ by only a few repeat units.

A deviation of the specificity of a cleavage site of a restriction endonuclease.

Caused by the substitution, deletion, or insertion of a single nucelotide.

A PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest.

Locus used by forensic DNA laboratories for AFLP analysis.

The _____ is used for forensic sex-typing applications.