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Taxonomy
science of classification of living organisms
consists of classification, nomenclature, and identification
Classification
arrangement of organisms into taxonomic groups (known as taxa)
genus
taxonomic rank used in the classification of living organisms. It is a group of species that share a set of characteristics and are closely related evolutionarily.
specific eptithet
second part of a scientific name used to identify a particular species within a genus.
written in lowercase letters.
Bacteria and archaea
The Five-Kingdom System of Classification:
Kingdom Prokaryotae
Algae and protozoa
The Five-Kingdom System of Classification:
Kingdom Protista
Fungi
The Five-Kingdom System of Classification:
Kingdom Fungi
Plants
The Five-Kingdom System of Classification:
Kingdom Plantae
Animals
The Five-Kingdom System of Classification:
Kingdom Animalia
Viruses
_ are not included in the The Five-Kingdom System of Classification because they are acellular.
Archaea and Bacteria
The Three-Domain System of Classification:
(prokaryotic)
Eucarya
The Three-Domain System of Classification:
(all eukaryotic organisms)
Three-Domain System of Classification
based on differences in the structure of certain ribosomal RNA (rRNA) molecules among organisms
Acellular Microbes
includes viruses, viroids, prions
also called "infectious particles”
virions
Complete virus particles
oncogenic viruses/ oncoviruses
viruses that cause specific types of cancer
examples: Epstein–Barr virus, human papillomaviruses, and human T-lymphotrophic virus type 1 (HTLV-1).
Properties of Viruses
possess either DNA or RNA
unable to replicate on their own
lack genes & enzymes
depend on the ribosomes, enzymes, and metabolites of the host cell for protein and nucleic acid production
Bacteriophages/ phages
Viruses that infect bacteria
virulent bacteriophages
categories of bacteriophages:
always cause what is known as the lytic cycle, which ends with the destruction of the bacterial cell.
temperate bacteriophages
also known as lysogenic phages
type of bacteriophage that can either replicate lytically (i.e., immediately killing the host bacterial cell and releasing new virions) or integrate their genome into the host cell's chromosome and become dormant, known as lysogeny.
Antiviral agents
drugs that are used to treat viral infections.
they interfere with virus-specific enzymes and virus production by disrupting critical phases in viral multiplication or inhibiting synthesis of viral DNA, RNA, or proteins.
Antibiotics
_ are not effective against viral infections
Human immunodeficiency virus (HIV)
virus causes acquired immunodeficiency syndrome (AIDS).
Prions
small infectious proteins that cause fatal neurologic diseases in animals and humans
most resistant to disinfectants.
Viroids
short, naked fragments of single-stranded RNA, which can interfere with the metabolism of plant cells.
Cocci
round bacteria
may be seen singly or in pairs, chains, clusters, packets of 4, or packets of 8
Bacilli
often referred to as rods; they may be short or long, thick or thin, and pointed or with curved or blunt ends.
occur singly, in pairs, in chains, in long filaments, or branched
coccobacilli
Extremely short bacilli
Heat fixation
two most common techniques of fixation:
not a standardized technique; excess heat will distort bacterial morphology
Methanol fixation
two most common techniques of fixation:
a standardized technique; the preferred method
Gram-positive bacteria
The cell walls of _ have a thick layer of peptidoglycan, making it difficult to remove the crystal violet–iodine complex.
Gram-negative organisms
_ have a thin layer of peptidoglycan, making it easier to remove the crystal violet; the cells are subsequently stained with safranin
fastidious
Organisms with especially demanding nutritional requirements
pathogenicity
_ is tested by injecting the organism into mice or cell cultures
Pathogens
_ are able to cause disease because they possess:
capsules, pili, or endotoxins
secrete exotoxins & exoenzymes
molecular diagnostic procedures
Methods used to identify bacteria in the lab are going toward looking at the organism's DNA or RNA.
Rickettsias, chlamydias, mycoplasmas
3 bacteria that do not posses all the attributes of typical bacterial cells
obligate intracellular pathogens
they must live within a host cell; they cannot grow on artificial culture media
ex. rickettsias and chlamydias
leaky membranes
Rickettsias have _
energy parasites
Chlamydias are _ meaning they prefer to use ATP molecules produced by their host cell
Mycoplasmas
Smallest of the cellular microbes
Lack cell wall (assume many shapes)
can cause atypical pneumonia and genitourinary infections in humans
resistant to drugs that attack cell walls
produce tiny “fried egg”
Photosynthetic bacteria
_ include purple bacteria, green bacteria, and cyanobacteria
all use light energy as an energy source
Purple and Green Bacteria
_ and _ do not produce oxygen
Cyanobacteria
_ bacteria that produces oxygen
Oxygenic photosynthesis
photosynthesis that produces oxygen
anoxygenic photosynthesis
photosynthesis that does not produce oxygen
Archaea
“ancient”
live in extreme environments
cell walls do not contain peptidoglycan
Algae
photosynthetic, eukaryotic organisms
produce by photosynthesis
arranged in colonies or strands
cell walls contain cellulose
Prototheca
rare genus of algae that cause human infections
phycotoxins
produced by dinoflagellates that cause “red tides” and can lead to
Protozoa
non-photosynthetic, eukaryotic organisms
unicellular, free-living, found in soil & water
pellicle
thickened cell membrane found in protozoa
trophozite
2 stages of protozoan life cycle:
motile, feeding, dividing stage
cyst
2 stages of protozoan life cycle:
nonmotile, dormant, survival stage
Pseudopodia
“false feet”
used by amebae for movement
hairlike cilia
used by ciliates for movement
whiplike flagella
flagellates move by means of_
Sporozoa
_ have no visible means of locomotion
mycology
study of fungi
chitin
polysaccharide found in fungal cell walls
hyphae
_ are the main mode of vegetative growth in fungi, and are collectively called a mycelium.
Budding, hyphal extension, formation of spores
_ _ _ are types of reproduction for fungal cells
septate hyphae
the hyphae are divided into cells by cross walls or septa
aseptate hyphae
the hyphae do not contain septa
Yeasts
_ are eukaryotic, unicellular organisms that lack
mycelia
blastospores or blastoconidia
Individual yeast cells, also referred _
can be observed only using a microscope
pseudohypha
A string of elongated buds (yeasts)
chlamydospores
Some yeasts produce thick-walled, spore-like structures called _
simple wet mount
_ can be used to differentiate yeast colonies from bacterial colonies.
Yeasts
larger than bacteria and are usually oval-shaped
observed in the process of budding.
mycoses
infectious diseases of humans and animals that are caused by moulds are called _
Superficial mycoses
_ are fungal infections of the outermost areas of the human bodyhair, nails, and epidermis.
Cutaneous mycoses
_ are fungal infections of the living layer of the skin, the dermis.
Subcutaneous mycoses
_ are fungal infections of the dermis and underlying tissues
Systemic mycoses
_ are fungal infections of the internal organs of the body
dimorphism
ability to live as either yeasts or molds, depending on growth conditions:
When grown in vitro at body temperature (37C), dimorphic fungi grow as yeasts and produce yeast colonies
When grown in vitro at room temperature (25C), dimorphic fungi exist as moulds, producing mould colonies.
five steps in the lytic cycle
Attachment, penetration, biosynthesis, assembly, and release
Latent virus infections
viral infections in which virus is able to hide from a host’s immune system by entering the cells and remaining dormant