Enzymes and Replication
Helicase
unwinds the parental double helix at replication forks
single-strand binding protein
binds to and stabilizes single-stranded DNA until it is used as a template
topoisomerase
breaks, and rejoins parental DNA ahead of replication fork, relieving the strain.
Primase
lays down the RNA primer (synthesizes)
DNA polymerase III
synthesizes a new DNA strand by adding a nucleotide to an RNA primer or a per-existing DNA strand.
DNA polymerase I
removes RNA primers and sticks in bases, replaces them with DNA nucleotides
Ligase
gap in the sugar phosphate backbone, will join okazaki fragments in lagging strand, and in leading strand will join 3’ end of DNA that replaces primer to the rest of leading strand DNA
Okazaki fragments
segments of lagging strand
Lagging strand
discontinuously synthesized DNA strand that elongates by means of okazaki fragments synthesized away from the replication fork
Leading strand
the new complementary DNA strand that is synthesized continuously along the template strand toward the replication fork
3’ - 5’ is ….
the leading strand
5’ - 3’ is…..
the lagging strand