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Helicase

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Enzymes and Replication

12 Terms

1

Helicase

unwinds the parental double helix at replication forks

<p>unwinds the parental double helix at replication forks</p>
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2

single-strand binding protein

binds to and stabilizes single-stranded DNA until it is used as a template

<p>binds to and stabilizes single-stranded DNA until it is used as a template</p>
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3

topoisomerase

breaks, and rejoins parental DNA ahead of replication fork, relieving the strain.

<p>breaks, and rejoins parental DNA ahead of replication fork, relieving the strain.</p>
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4

Primase

lays down the RNA primer (synthesizes)

<p>lays down the RNA primer (synthesizes)</p>
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5

DNA polymerase III

synthesizes a new DNA strand by adding a nucleotide to an RNA primer or a per-existing DNA strand.

<p>synthesizes a new DNA strand by adding a nucleotide to an RNA primer or a per-existing DNA strand.</p>
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6

DNA polymerase I

removes RNA primers and sticks in bases, replaces them with DNA nucleotides

<p>removes RNA primers and sticks in bases, replaces them with DNA nucleotides</p>
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7

Ligase

gap in the sugar phosphate backbone, will join okazaki fragments in lagging strand, and in leading strand will join 3’ end of DNA that replaces primer to the rest of leading strand DNA

<p>gap in the sugar phosphate backbone, will join okazaki fragments in lagging strand, and in leading strand will join 3’ end of DNA that replaces primer to the rest of leading strand DNA</p>
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8

Okazaki fragments

segments of lagging strand

<p>segments of lagging strand</p>
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9

Lagging strand

discontinuously synthesized DNA strand that elongates by means of okazaki fragments synthesized away from the replication fork

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10

Leading strand

the new complementary DNA strand that is synthesized continuously along the template strand toward the replication fork

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11

3’ - 5’ is ….

the leading strand

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12

5’ - 3’ is…..

the lagging strand

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