Linkage Mapping Via Crosses

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RFLP

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26 Terms

1

RFLP

________: is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes.

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2

SNP

________: are a type of polymorphism involving variation of a single base pair.

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3

Allelic differences

________ between genes were used to map the relative locations of those genes along a chromosome by conducting test crosses.

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4

Molecular markers

________ are polymorphic; within a population, they may vary from individual to individual.

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5

diploid species

In a(n) ________, an individual has 2 copies of a given STS.

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6

STS

________: Stretch of DNA that a PCR can easily detect.

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7

Microsatellites

________: short repetitive sequences that are abundantly interspersed throughout a species genome and tend to vary in length among different individuals.

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8

AFLP

________: Same as RFLP nut the DNA sequence being used is amplified via PCR instead of isolating the chromosomal DNA.

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9

Distances

________ between linked molecular markers determine the outcome of crosses.

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10

DNA fragment

The ________ in the higher band is more extended because it has more repeat sequences than does the lower band.

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11

GH1

________: encodes acetolactate synthase.

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12

DNA

When the ________ is collected from a haploid cell, an STS produces only a single band.

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13

pair of PCR

If a(n) ________ primers copies a single site within a set of chromosomes, the amplified region is called a Sequence Tagged Site.

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14

STS

When a(n) ________ contains a microsatellite, the PC products may be identical and result in a single band on a gel if the region is the same length in both copies.

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15

Introduction

Definitions

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16

Mapping

the experimental process of determining the relative locations of genes or other segments of DNA on individual chromosomes

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17

Molecular marker

Segment of DNA that is found at a specific site along a chromosome and has properties that enable it to be uniquely recognized using molecular tools

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18

RFLP

is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes

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19

AFLP

Same as RFLP nut the DNA sequence being used is amplified via PCR instead of isolating the chromosomal DNA

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20

SNP

are a type of polymorphism involving variation of a single base pair

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21

STS

Stretch of DNA that a PCR can easily detect

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22

RFLPs

Restriction fragment length polymorphism

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23

Atc4

encodes actin

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24

er

encodes erecta

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25

GH1

encodes acetolactate synthase

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26

Microsatellites

short repetitive sequences that are abundantly interspersed throughout a species genome and tend to vary in length among different individuals

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