Module 2 (MOLBIO LAB)

All organisms contains both DNA and RNA except for?

___________ are our genetic information.

Central Dogma of Molecular Biology: DNA - DNA

Central Dogma of Molecular Biology: DNA - RNA

Central Dogma of Molecular Biology: RNA - Proteins

Information from ____ are not directly utilized.

Transcripts must be made from DNA to produce the genetic information, these are?

is double-stranded, forming a double helix.

usually single-stranded

The double helix, the twisted-ladder structure of deoxyribonucleic acid (DNA) was discovered by?

____ are polymers of nucleotides.

COMPONENTS OF NUCLEIC ACID

What do you call the double stranded RNA virus?

What do you call the sugar in DNA?

What do you call the sugar in RNA?

What do we call a stucture containing 5 carbons?

The phosphate group is attached to the pentose sugar (DNA/RNA) via the ___ by a _____.

A nitrogenous base is attached to the pentose sugar (DNA/RNA) via the 1’ carbon by a _____

What are the two main bases?

9 Carbon atoms; Double ring

What are the two substituted purines?

6 carbon atoms; single ring

What are the three substituted pyrimidines?

Base Pairing: Adenine and? (DNA)

Base pairing: Adenine and? (RNA)

Base pairing: Guanine and?

A nitrogenous base with a pentose sugar component.

Nucleoside is a combination of?

Nucleoside differs from nucleotide because it lacks?

A substance made up of DNA or RNA and proteins,

Proteins that binds DNA in the nuclei of eukaryotic cell

One of the two identical halves of a chromosome that has been replicated in preparation for cell division

Bundles of tightly coiled DNA located within the nucleus of almost every cell in our body

It links a pair of sister chromatids together during cell division

DNA strands are long hence they are condensed and wrapped around proteins called _______ to form ___________.

Chromatin are still long and further condensed to form ____________________

SOURCES OF DNA

Purpose of Extraction: to release ____ from the cell

Released nucleic acid from the cell can be used for analysis such as?

Analysis blot: Southern

Analysis blot: Northern

Analysis Blot: Western

What are the 3 methods for the isolation of DNA?

What are the 2 methods for the isolation of RNA?

In Organic Isolation Method of DNA, cells are lysed to form _____, which releases the nucleic acid in the cells.

In Organic Isolation Method of DNA, ___ and ____ are used for the lysis of cells.

SDS stands for?

What are the contaminants in Organic Isolation Method of DNA?

Contaminants in Organic Isolation Method of DNA are removed by adding _____.

Phenol-Chloroform Mixture: Organic Compound or Inorganic Compound

In Organic Isolation Method of DNA, DNA in aqueous solution will float on top. Which will be placed on a new tube and precipitated using ___ or ____ then centrifuged.

In Inorganic Isolation Method, _______, _____ and ____ are used for the lysis of cells.

EDTA stands for?

What is the function of Tris or tris(hydroxymethyl) aminomethane in Inorganic Isolation Method of DNA?

Contaminants in Inorganic Isolation Method of DNA must be removed by ________

The supernatant containing the DNA is then mixed with ___ or ____to precipitate the DNA to be collected and resuspended in a smaller volume. (Inorganic Isolation Method)

In Solid Phase Isolation Method, DNA solution are placed in a _____ which is a solid matrix.

In Organic Isolation Method of RNA, the RNA must be protected from intracellular and extracellular RNases using _____

What should be done to remove any contaminating DNA in an RNA solution?

In Solid-Phase Isolation Method of RNA, what is added to the spin column to destroy remaining DNA?