What is a cell
The basic structural and functional unit of every organism
All cells are… (4 things)
Bound by a plasma membrane
Contain cytoskeleton
Contain chromosomes
Contain ribosomes
What are the two types of cells and their defining features
Prokaryotes - consists of the domains bacteria and archaea, dna is in the nucleotide region, generally smaller than eukaryotes
Eukaryotes - consists of the domains protists, fungi, animals, and plants, dna is in the nucleolus, and they contain membrane bound organelles
Organelles
Membrane bound structures in eukaryotes
Two classifications of organelles
Endomembrane organelles - nuclear envelope, endoplasmic reticulum, golgi complex, lysosomes, vesticles/vacuoles, plasma membrane
Energy organelles - mitochondria, chloroplasts
What is compartmentalization and what are the two benefits
Allows for different metabolic reactions to occur in different locations.
Increases surface area for reactions
Prevents interfering reactions from occurring in the same location
What are the unique cell components in plant and animal cells
Plants - chloroplasts, central vacuole, cell wall, plasmodesmata
Animals - lysosomes, centrosomes, flagella
Nucleus (all of its details)
Contains the genetic information (chromosomes) and is enclosed by the nuclear envelope
Has a double membrane which has pores - regulate entry and exit of materials
Contains a nucleolus
Nucleolus
The dense region where ribosomal rna is synthesized
RRNA is combined with proteins to form large and small sub units of ribosomes
Ribosomes
Comprised of ribosomal RNA and protein
Function: synthesize protein
Where are ribosomes found
Cytosol “free ribosomes”
Endoplasmic reticulum “bound ribosomes”
Endoplasmic reticulum
Functions - Synthesizes membranes and compartmentalizes the cell to keep proteins formed in the rough ER separate from the free ribosomes
Rough ER
Contains ribosomes bound to its membrane
Smooth ER
Contains no ribosomes, synthesizes lipids, metabolizes carbohydrates, and detoxifies the cell
Golgi Complex/Apparatus
Contains flattened membranous sacs Called cisternae and separates them from the cytosol
Does the golgi complex have directionality
Yes - has two faces - the cis and trans face
Cis face - receives vesicles from the ER
Trans face - sends vesicles back out into cytosol to other locations or to the plasma membrane for secretion
Function of the Golgi Complex
Receives transport vesicles with materials from the ER
Modifies the materials - ensures Newley folded proteins are folded correctly
Sorts the materials
Adds molecular tags
Packages materials into new transport vesicles that exit the membrane via exocytocis
Lysosomes
Membranous sac with hydrologic enzymes
Function - hydrolyzes macromolecules in animal cells
Autophagy - lysosomes can recycle their own cells organic materials which allows the cell to renew itself
Peroxisomes
Similar to lysosomes - membrane bound metabolic compartment
Catalyses reactions that produce H2O2 and they have specific enzymes which break down the H2O2 into H2O
Vacuoles - list the three and their function
Large vesicles that stem from the ER and Golgi. They are selective in transport
Food vacuole - formed via phagocytosis (cell eating) and then are digested by the lysosomes
Contractile Vacuole - Maintain water levels in cells
Central Vacuole - Found in plants
Contains inorganic ions and water
Maintains turgor pressure
Mitochondria
Site of cellular respiration “Power house of the cell”
Has a double membrane
Inner membrane is smooth and has folds called Cristal
Divides the mitochondria in to two internal compartments and increases the surface area
Mitochondrial matrix
Is enclosed by the inner membrane (the space between the inner and outer membrane)
Where the Krebs cycle happens
Contains enzymes that catalyze cellular respiration and produce ATP, mitochondrial DNA, and ribosomes
The number of mitochondria in a cell correlated with what
Metabolic activity
Cells with high metabolic activity have more mitochondria
Chloroplasts
Specialized organelles in photosynthetic organisms
Site of photosynthesis
Contains green pigment chlorophyll
Double membraned
What is found inside the chloroplast double membrane
Thylakoids that are organized into stacks called grana and is surrounded by the fluid stroma that contains chloroplast DNA, ribosomes, and enzymes
Where do light dependent reactions occur?
The grana
Where is the location of the Calvin cycle
The stroma
What is the endosymbiosis theory
Explains the similarities between mitochondria and chloroplasts to prokaryotes
States that early eukaryotic cell engulfed prokaryotic cell
Endosymbiosis evidence
Double membrane, unique dna, ribosomes (all found within the mitochondria and chloroplasts)
the organelles are able to function on their own
The cytoskeleton
A network of fibers throughout the cytoplasm
Gives structural support and mechanical support
Anchors the organelles
Allows for movement
What are the three fibers in the cytoskeleton
Microtubules, microfilaments, and intermediate filaments
Microtubules
Hollow rod structures made of the protein tubulin
Grows from the centrosome
Assists in chromosomal separation during cell division
Helps with motion and structural support
Microfilaments
Thin solid rods made of the protein actin
Function is to maintain cell shape my bearing tension, assisting in muscle contraction, and division of animal cells (contractile ring of the cleavage)
Intermediate filaments
Fibrous proteins made up of varying subunits that are permanent elements of the cell
Function is to maintain cell shape, anchor nucleus and organelles, and it forms the nuclear lamina that lines the nuclear envelope
Calvin cycle
The Calvin cycle is the second half of photosynthesis, the light independent reaction, that converts co2 into glucose/energy
Krebs cycle
The process that happens in the mitochondria that uses glucose which produces ATP and gives out water and carbon dioxide as byproducts