Chapter 14: Identification of Semen

The process of generating spermatozoa.

A typical ejaculation releases _____ of semen, which contains seminal fluid and sperm cells.

A normal sperm count ranges from _____ spermatozoa per milliliter of semen.

is a complex mixture of glandular secretions.

____ account for approximately 30% of the ejaculate.

The surgical removal of a bilateral segment of the ductus deferens. It prevents spermatozoa from reaching the distal portions of the male p[[[[[[eproductive tract.

The condition by which males have abnormally low sperm counts.

A condition that causes males to produce no spermatozoa.

The ________ contains a nucleus with densely packed chromosomes.

A membranous compartment containing enzymes essential for fertilization found at the tip of the head.

The _________ is responsible for spermatozoon motility.

consists of a group of phosphatases with optimal activity in an acidic pH environment.

It is a major protein present in seminal fluid at concentrations of 0.5–2.0 mg/mL.

Human __________ includes two major types, semenogelin I (SgI) and semenogelin II (SgII).

The AP contained in semen can hydrolyze a variety of phosphate esters. It catalyzes the removal of the phosphate group from a substrate. An insoluble colored precipitate at sites of acid phosphatase activity is formed with a stabilized diazonium salt.

These are more sensitive than the colorimetric detection of AP and are used for semen stain mapping. AP catalyzes the removal of the phosphate residue on a 4-methylumbelliferone phosphate (MUP) substrate a reaction that generates fluorescence under ultraviolet light.

The cells from a questioned stain on an absorbent material can be transferred to a microscope slide by extracting a small portion of a stain with water, followed by gentle vortexing.

A dye used for staining the nuclei of spermatozoa in the presence of aluminum ions.

A green component that stains the neck and tail portions of the sperm.

_______ has been shown to be an effective technique for separating spermatozoa from nonsperm cells.