PCR + Electrophoresis
PCR
There’s a target sequence in the Dna that we’re trying copy
the target sequence is a gene
creates a large amount of copies
Denature
done at 94 degrees Celsius
done to pull apart the the Dna strands
Annealing
done at 40-65 degree Celsius
The stage where the primer attaches to the strands if dna
identifies the start and the end of the target sequence
Extension
occurs at 72 degrees Celsius
stage where taq polymerase attaches to the strands
taq polymerase builds the dna compliment of target sequence using the dnpt’s
dnpts are the free dna nucleotides
Thermocyler
the machine used for pcr
Electrophoresis
Pouring the gel
Preparing your samples
Loading the gel, 4) Running the gel (exposing it to an electric field)
Dna is negative because of the sugar phosphate backbone it has
Staining the gel.
PCR + Electrophoresis
PCR
There’s a target sequence in the Dna that we’re trying copy
the target sequence is a gene
creates a large amount of copies
Denature
done at 94 degrees Celsius
done to pull apart the the Dna strands
Annealing
done at 40-65 degree Celsius
The stage where the primer attaches to the strands if dna
identifies the start and the end of the target sequence
Extension
occurs at 72 degrees Celsius
stage where taq polymerase attaches to the strands
taq polymerase builds the dna compliment of target sequence using the dnpt’s
dnpts are the free dna nucleotides
Thermocyler
the machine used for pcr
Electrophoresis
Pouring the gel
Preparing your samples
Loading the gel, 4) Running the gel (exposing it to an electric field)
Dna is negative because of the sugar phosphate backbone it has
Staining the gel.