Sedimentation 

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particle

A(n) ________ is accelerating till the coulombs force is equal to the frictional force.

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Isoelectric focusing

A) ________: separation of proteins based on isoelectric points (pH value where they have no net charge), it is the first step in 2D electrophoresis.

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sedimentation constant

The ________ gives the moving velocity of a particle at certain centrifugal acceleration.

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Centrifugal force

inertial force in a rotating system, it is radial from the center and equal to centripetal force

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Buoyancy

depends on volume immersed in liquid, density of the liquid and acceleration

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Drag force

resistance force caused by movement of a body (transferring energy to molecules) through a fluid

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a. Preparative

separation of molecules based on size and mass of molecules

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b. Analytical

determination of size and mass of particles

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Agarose gel

separating nucleic acid based on charge

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10
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SDS PAGE

separation of protein based on its size using a vertical tank

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a) Isoelectric focusing

separation of proteins based on isoelectric points(pH value where they have no net charge), it is the first step in 2D electrophoresis

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b) 2D electrophoresis

the proteins are then separated based on their molecular weight by SDS-PAGE

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