For fluorescent-antibody source of illumination that causes fluorescent compounds in a specimen to emit light.
A single photon is used to illuminate a specimen at a time.
Two photons are used to illuminate a living cell.
A sound wave is used to look at living cells attached to frequencies that travel through a specimen to another surface.
The beam of electrons is used to examine the viruses or the light, instead of the internal ultrastructure, because of the shorter sections of cells.
The beam of electrons is used to study the surface features of light, instead of reflecting electrons from the specimen.
A thin metal probe scans a specimen and produces an image inside a cell.
The surface of the specimen is gently forced down along the three-dimensional images.
A nearly three-dimensional image is produced.
The cocci and rods are both positive.
The Gram stain and the acid-fast stain are the most frequently used differential stains.
Acid dyes include eosin, acid fuchsin, and nigrosin.
A mordant is covered with a chemical to intensify the solution.
One function of a mordant is to increase the affinity of a stain for a biological specibacteria.
The sample is washed again, and this time it is found to be stained with gram-negativebacteria.
The gram-positivebacteria retain de-ah.
In the acid-fast staining procedure, the red dye carbolfuch is applied to a fixed smear and the slide is gently heated to react differently to the Gram stain because of structural dif for several minutes.
The slide is washed with a mixture of crys water and crystal violet.
The izer removes the red stain frombacteria that are not gram-positive, but have a thicker peptidoglycan cell wall acid.
The acid-fast microorganisms retain the pink and the gram-negativebacteria do not.
When applied to gram-positive and gram-negative cells, crystal violet and iodine enter the cells.
CV-I is formed by the combination of the crystal vio let and iodine.
The peptidogly of the electron microscope is much larger than that of a light microscope.
It provided irrefutable proof of the presence of spiralbacteria.
Maryanne's gram-negative cells are pink after she prescribes the antibiotic with safranin.
One week after finishing purple, gram-positive cells retain the dye.
Maryanne's Gram-negative cells do not retain the dye; they are treated with a red dye.
Light microscopy and a Gram stain are used to view the Gram reaction in a lab.
penicillins and cephalosporins can be used to kill gram-positive bacte ria.
The antibiotics can't penetrate the lipopolysaccharide layer so gram-negativebacteria are more resistant.
Maryanne's physician prescribes antibiotics when she suspects thebacteria are resistant to clarithromycin.
She feels like her old self again and is back in the office full time.
The methy capsule staining is more difficult than other types of counterstain.
The non-acid-fast cells appear blue after the procedures.
The flagella are thicker in relation to the body of the cell because of the stain that has accumulated from the mordant.
It is used to distinguish different types ofbacteria.
Gram-positive and gram-negative are the two large groups of the bacteria.
The crystal violet stain is retained by gram-positivebacteria.
Gram-negativebacteria do not retain the crystal violet stain, they are counterstained with safranin and then appear pink.
Sometimes used as a diagnostic aid, it is used to color and isolated various structures.
It was used to show the presence of a capsule.
The capsules are unstained and stand out against a contrasting background because they do not accept most stains.
It can be used to detect the presence of organisms.
When malachite green is applied to a heat-fixed smear of cells, it stains them green.
The rest of the cells are either red or pink after safranin is applied.
It was used to show the presence of flagella.
Malachite green, the eters of the flagella until they become visible under the light primary stain, is applied to a heat-fixed smear and heated to the microscope.
The number is used by microbiologists for about 5 minutes.
The heat helps the stain pen and arrangement of flagella.
Next, we will take a closer look at the structure of microbes and how they protect, nourish, and reproduce themselves.