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6 -- Part 5: Microbial Growth

- The loop lated colonies.
- The colonies can be picked up with an inocu.
- In the second and third series, the loop picks the lating loop and transfers it to a test tube to culture a single type of bacterium.

- There are many variations of this pattern.

- When the organ types are red and yellow, the streak plate method works well.

- Humans on the Space Station would die instantly from cold and the vacuum of space if it were to suddenly burst.

- Direct and indirect methods of measuring cell temperature can be used.

- It's important to be able to determine tures from 250 to 295degC.
- The culture can usu numbers directly by counting or indirectly by ally, which can be thawed and cultured several years later.
- They measured their metabolism activity.

- While under vacuum, the container is sealed by melting the rial growth, not a glass with a high-temperature torch.
- The size of the individual cells increases.

- The organisms can be revived by hydration with a liquid medium.

- The cells are replicated.

- When the number of cells in each genera tion is expressed as a power of 2, the exponent tells the number of doublings that have occurred.

- It varies depending on the environment and the organisms.

- A generation time of 1 to 3 hours is the average for mostbacteria.
- An enormous number of cells will be produced.

- 25 is the number of generations indicated by the superscript.

- To arrive at the numbers in the center column, certain actinomycetes reproduce by using the yx key on your calculator.
- The number 32 will be shown on the calculator.
- A few colonies ofbacteria will have 32 cells.
- The log key on your calculator can be used to arrive at the numbers in the right-hand column.
- Simply fragment, and the fragments initiate the growth of the number 32, then press the log key.
- The rounded cells will be shown by the calculator.

- It's hard to graph population changes using numbers.

- The graph logarithmic plot point for the tenth generation (3.01) has been drawn so that the curves intersect up the graph.
- The figure shows how graphs of changes in the number ofbacteria would increase the plots rather than decreasing them.
- Take the line off the page if it's at ten height of the graph.

- If the numbers were plotted for two more generations.

- We are not used to it and the cells are not active.
- It is necessary for a period of intense metabolic activity involving, in proper understanding of graphs of microbial populations, that the population is thinking in logarithmic relationships.

- The lag, log, which the population doubles, is one of the four basic phases of growth.

- For a while, the number of cells changes very little because of the ferred for industrial purposes where, for example, a product cells do not immediately reproduce in a new medium.

- Knowledge of the growth curve is important to understand population dynamics and population control in the course of infectious diseases, as well as in food preservation and spoilage.

- Microbial death will be discussed in Chapter 7 if exponential growth continues.

- The population curve would be the same as that of an 80,000-ton aircraft carrier if two mice started a family with a fixed food supply.

- It's not always clear what causes exponential growth to stop.

- The amount of waste products is proportional to cell numbers.
- Population numbers are related to changes in the pH.

- In the pop example, a millionth of a liter of sour milk is diminished to a tiny fraction of the number of cells.
- There must be 70 times in the previous phase until the population dies out.

- In serial dilutions, the original inoculum is changed in a series of tubes.
- Each dilution tube will only have a fraction of the number of cells as the preceding one.
- colonies can be counted using samples of the dilution used to inoculate the Petri plates.
- The count is used to estimate the number ofbacteria in the sample.

- It's not practical to measure out a millionth of a millionth of a gram of food in some applications.
- It's not possible to hold a particular lot for this length of time.

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