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17.7 Recombinant DNA
The replacement of leucine with alanine in the UCA with adenine is the result of a point mutation in the mRNA.
How would the substitution affect the molecule?
Human growth hormone, interferon, and blood clotting factor VIII are some of the important medicines that are produced using the technology of recombinant DNA.
The same restriction enzymes are used to cut a piece of DNA called donor DNA from a gene of a different organisms.
As the recombined cells divide and replicate, they produce theProtein from the inserted gene in the plasmids.
Try Practice Problems 17.69 shows some of the products developed through the use of recombinant DNA technology.
A recombinant DNA new gene can be formed by placing a Gene from another organisms in a plasmid DNA donor, which causes the same restriction to produce a nonbacterial enzyme.
Number of copies of the DNA section was a process in 1987.
The genes are heated in a short time.
The DNA is heated to separate the strands.
Screening for genes with known sequence is possible with the help of the PCR.
There are several defects in two breast cancer genes that correlate to a higher risk of breast cancer.
Patients are screened for the defects in their genes by taking a sample of their saliva or blood.
If the test is positive, a fluorescent label will be visible.
Unless there are identical twins, the chance of two people of the same ethnic background having the same 13 genetic traits is 1 in 575 trillion.
A very small sample size is needed from semen.
During the process of amplification, radioactive or fluorescent isotopes are incorporated.
The DNA is cut into smaller pieces by restriction enzymes, which are placed on a gel and separated.
The gel has a pattern called a DNA fingerprints.
In forensic science, a person's fingerprints can be used to connect a suspect to a crime, if they have a sample of their own.
A crime has been solved with the use of DNA fingerprints.
The Human Genome Project showed that our genes are composed of 3 billion bases and 21 000 genes.
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