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10 -- Part 5: Classification of Microorganisms
The strain was grown over the entire plate.
The areas of lysis were produced by the phages, indicating that the strain was treated with the same restriction.
The percentage of guanine plus cytosine is expressed as usu al y.
A comparison of the G 1 C content in different species can reveal the degree of species relatedness.
The guanine and the C are in the same place in the DNA.
Each adenine in the DNA has a different mine.
The percentage of DNA bases that are GC pairs also tells us the percentage of AT pairs.
Two organisms with similar genes will have the same amount of bases in their DNA.
The same restriction enzyme was used to digest the same amount of GC from seven differentbacteria.
The digests were put in two different organisms.
There are two organisms in the agarose gel.
An electrical current with the same percentage of GC isn't necessarily applied to the gel to separate the fragments by size and charge.
The lanes have a comparison of the samples.
It is possible to determine the source of hospital-acquired infections.
The hospital was able to break the infection's chain of identifies because Monica and her friend encouraged the nurse to use aseptic techniques.
If a double-stranded molecule of DNA is subjected to heat, the can be used to increase the amount of microbial DNA to levels that can be tested by gel electrophoresis.
Between the bases break, NAATs use PCR.
If the single strands are cooled.
The presence of a primer for a specific microorganism is the same as the original double strand.
In 1907, organisms described Whipple's disease.
The procedure assumes that if two species are similar or related, the order is caused by an unknown bacillus.
No one has been able to duplicate the majority of their nucleic acid sequence.
Between any single amplify DNA coding for rRNA in the amber, there can be similar hybridization reactions.
The strands of the nucleic acid chain are stranded.
The basis of several techniques is the sequence of the DNA.
The below information was used to determine the relationships between ancient and modernbacteria.
The identification was made in a record time, less than 2 weeks.
One method involves breaking the DNA.
A new strain of H7N9 influenza virus must be hybridized with this fragment.
The organisms are more closely related when there is more than one strand of DNA from them.
To separate strands, heat.
It's cool to allow renaturation of double-stranded DNA.
The cloned fragment is from the collected fragment.
The cells are lysed.
Cloned DNA fragments are marked with fluorescent dye and separated into single strands.
The strands of DNA are separated.
The Fluorescent probe is added to the DNA.
The excess probe is washed off.
The chip contains probes.
A fluorescent dye is added to the chip to make it look like it contains hundreds of thousands of organisms.
Each DNA sequence is assumed to be fluorescent.
There are several advan tags.
All cells have ribosomes.
The same "signature" sequence in their rRNA is separated into single strands.
The rRNA was labeled with a fluorescent dye.
Cells don't have to be cultured in the laboratory in order to benefit from the third advantage.
The rRNA primer can be used to amplify the DNA.
The amplified fragments are cut with one or more restriction enzymes.
The band patterns can be compared.
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