6: Enzymes Equations

What is Vo in terms of Vmax?

Vo = Vmax*[S] / (Km + [S])

What is Vo in terms of kcat?

Vo = kcat*[Et][S] / (Km + [S])

What is kcat equal to?

kcat = Vmax / [Et]

What is Vo during competitive inhibition?

Vo = Vmax*[S] / (αKm+[S]) OR Vo = kcat*[Et][S] / (αKm + [S])

What is Vo during uncompetitive inhibition?

Vo = Vmax*[S] / (Km + α'[S]

What is α equal to?

1 + [i]/Ki where i is the inhibitor

What is a' equal to?

1 + [i]/K'i where i is the inhibitor

What is Ki equal to?

Ki = [E][I] / [EI]

What is Ki' equal to?

Ki' = [ES][I] / [ESI]