Competency Review Advanced Micro 3

Colony and microscopic morphology of: Penicillium

Microscopic: Septate hyphae, branched conidiophores with 2 branches, flask shaped phialides with chains of round conidia - brush like Colony: flat, powdery, blue green with white edge

Colony and microscopic morphology of: Fusarium,

Microscopic: septet hyphae, large multi-celled sickle shaped macroconidia on simple conidiophores. small 1-2 celled tear-drop shaped microconidia Colony: flat, pink to violet with light edge

Colony and microscopic morphology of: Gliocladium

Microscopic: Branching conidiophores with flask-shaped phialides like penicillium, microconidia cluster in ball like a golf ball Colony: White at first, center becomes dark green, reverse: white

Colony and microscopic morphology of: Paecilomyces

Microscopic: branching conidiophores with flask-like phialides in pairs or brush-like groups, long chains of oval microconidia, resembling penicillium Colony: yellowish brown, pink, white with reverse off-white, light yellow, pale brown

Colony and microscopic morphology of: Bipolaris

Microscopic: elongated conidiophores, oblong conidia with 3 to 5 separation Colony: Gray, brown, or black with brown. Reverse black.

Know the names of the structures found in Penicillium

Septate hyphae, branched conidiophores w/ secondary branches, flask shaped phialides w/ chains of round conidia

Know the names of the structures found in Fusarium

Septate hyphae, large multi-celled sickle-shaped macroconidia on simple conidiophores, small 1-2 celled tear-drop-shaped microconidia

Microscopic morphology of: Alternaria

septate hyphae and dark large conidia, club shaped

Microscopic morphology of: Curvularia

Septate hyphae Large conidia with up to 4 cells central cell swollen - curved

Microscopic morphology of: Scopulariopsis

short conidiophores with round, rough chains of conidia

Gram stain results for: Bacillus anthracis

Gram Positive Rod

Gram stain results for: Francisella tularensis

Tiny Gram Negative Coccobacillus

Gram stain results for: Yersinia pestis

Gram Negative Rods - may be bipolar stained

The colony morphology of Bacillus anthracis

Aerobic, 4-8hr growth No growth on MacConkey (GPR) Flat colonies with irregular edges Non-hemolytic on BAP Sticky colonies

Which of these are you most likely to come across in the southwest?

Yersinia pestis (plague)

Know the diseases caused by each organism: Bacillus Anthracis

Anthrax

Know the diseases caused by each organism: Francisella Tularensis

Tularemia

Know the diseases caused by each organism: Yersinia pestis

Plague

Things to consider determining if you have a bioterrorism organism

Patient history/travel history Symptoms Gram stain results - GP, small GNC, faintly staining GNR Growth and/or no growth on routine culture plates - Slow growth on blood agar plate - Slow or no growth on MacConkey plate