Competency Review Advanced Micro #2

How do we identify a stool pathogen on culture media?

NLF on MAC NLF on MAC Sorbitol Green or black colonies on HEK Growth on Campy CVA media for Campylobacter

Why do we need to use MacConkey Sorbitol? How do colonies appear on the media?

To identify E. Coli 0157:H7. The colonies appear NLF and colorless.

What organism grows on mannitol salt? Staph aureus vs. Coag neg Staph on plate

On MSA, Staph aureus (ferments mannitol) appear yellow while Coag negative staph (does not ferment mannitol) appear red.

Structures used to identify bacterial vaginosis?

Clue cells = Epi cells with bacteria

Dermatophytes microscopic & colony ID: Microsporum gypseum. Label the structures found in dermatophyte microscopic image

Microsporum Gypseum - Macroconidia

Dermatophytes microscopic & colony ID: Trichophyton rubrum. Label the structures found in dermatophyte microscopic image

Trichophyton Rubrum: Microconidia

Dermatophytes microscopic & colony ID: Trichophyton Mentagrophytes. Label the structures found in dermatophyte microscopic image

Trichophyton mentagrophytes

Klebsiella oxytoca: colony morphology, TSI, indole, motility

Colony morphology – MAC: LF, mucoid colonies. BAP: non-hemolytic, mucoid, cream to yellow colonies. GS - Gram negative Rods TSI – A/A + G Indole – positive Motility – negative

E. coli: colony morphology, TSI, indole, motility

Colony morphology – MAC: LF, mucoid colonies. BAP: beta hemolytic, sheen mucoid colonies. GS - Gram negative Rods TSI – A/A + G Indole – positive Motility – positive

Cornmeal microscopic: C. albicans

C. Albicans: clusters of blastospores along pseudohyphe

Cornmeal microscopic: C. tropicalis

C. Tropicalis: blastospores singly along pseudohyphae

Cornmeal microscopic: C. parapsilosis

C. Parapsilosis: curved pseudohyphae w blastoconidia singly or in clusters

Cornmeal microscopic: G. capititum

Geotrichum capitatum: Arthrospores squared-off ends and no blastospores.

Label phialide, vesicle, conidia, conidiophore on fungus

Phialide : vaselike structure that produces conidia Vesicle is what phialide comes out of.

Identify microscopic: Scedosporium

Scedosporium: septate hyphae, simple conidiophores with single oval conidia at the end

Identify microscopic: Sporothrix (different growth of Sporothrix)

Sporothrix: growth at 22 C will be mold (thin delicate hyphae with rosette conidia at end) while growth at 37 C will be yeast (Cigar bodies).

Aspergillus fumigatus microscopically and macroscopically

Aspergillus fumigatus: Microscopically (septate hyphae, conidiophore, phialides on the vesicle) (1 phialide on ½, or ⅓ of the vesicle). Macroscopically (flat, smoky gray, green w white edge, powdery)

How do Aspergillus terreus differ microscopically and macroscopically

Aspergillus terreus Microscopically (septate hyphae, rough conidiophore with 2 phialides in sunburst arrangement) Macroscopically (flat, yellow with white edges) (lines on front of plate)